BST2 Antikörper

Produktnummer ABIN7836143

Der Ratte Monoklonal anti-BST2 Antikörper (Klon 927) (ABIN7836143) detektiert spezifisch BST2 in FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für BST2 Antikörper (ABIN7836143)

Target: BST2 (Bone Marrow Stromal Cell Antigen 2 (BST2))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser BST2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 927

Produktdetails anti-BST2 Antikörper

Verwendungszweck: Rat monoclonal antibody to CD317

Spezifität: Recognizes mouse CD317

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Mouse plasmacytoid dendritic cells (DCs)

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Details zu BST2

Target: BST2 (Bone Marrow Stromal Cell Antigen 2 (BST2))

Andere Bezeichnung: BST2

Hintergrund: Bone marrow stromal antigen 2, BST-2, HM1.24 antigen, Tetherin, CD317

Gen-ID: 69550

Pathways: Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response