CD209b Antigen (CD209B) Antikörper

Produktnummer ABIN7835948

Der Armenischer Hamster Monoklonal anti-CD209b Antigen Antikörper (Klon 22D1) (ABIN7835948) detektiert spezifisch CD209b Antigen in FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für CD209b Antigen (CD209B) Antikörper (ABIN7835948)

Target: CD209b Antigen (CD209B)

Reaktivität: Maus

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 22D1

Produktdetails

Verwendungszweck: Armenian Hamster monoclonal antibody to CD209B

Spezifität: Recognizes mouse CD209B

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Partial mouse CD209B recombinant protein (74-325 aa)

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Antigendetails

Target: CD209b Antigen (CD209B)

Andere Bezeichnung: CD209B

Hintergrund: CD209 antigen-like protein B, DC-SIGN-related protein 1, DC-SIGNR1, OtB7, CD209

Gen-ID: 69165