Fc Receptor, IgG, Low Affinity IV (FCGR4) Antikörper

Produktnummer ABIN7835824

Der Armenischer Hamster Monoklonal anti-Fc Receptor, IgG, Low Affinity IV Antikörper wird verwendet zum Nachweis von Fc Receptor, IgG, Low Affinity IV in Proben von Maus. Er wurde validiert für FACS und IF.

Kurzübersicht für Fc Receptor, IgG, Low Affinity IV (FCGR4) Antikörper (ABIN7835824)

Target: Fc Receptor, IgG, Low Affinity IV (FCGR4)

Reaktivität: Maus

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 9E9

Produktdetails

Verwendungszweck: Armenian Hamster monoclonal antibody to CD16.2

Spezifität: Recognizes mouse CD16.2

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: FCgammaR4 -EC domain fusion with IgG1 Fc

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Antigendetails

Target: Fc Receptor, IgG, Low Affinity IV (FCGR4)

Andere Bezeichnung: FCGR4

Hintergrund: Low affinity immunoglobulin gamma Fc region receptor IV, CD16-2, FcgammaRIV

Gen-ID: 246256