ICAM2 Antikörper (FITC)

Produktnummer ABIN7835569

Der Ratte Monoklonal anti-ICAM2 Antikörper wird verwendet zum Nachweis von ICAM2 in Proben von Maus. Er wurde validiert für FACS und IF.

Kurzübersicht für ICAM2 Antikörper (FITC) (ABIN7835569)

Target: ICAM2 (Intercellular Adhesion Molecule 2 (ICAM2))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser ICAM2 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 3C4

Produktdetails anti-ICAM2 Antikörper (FITC)

Verwendungszweck: Rat monoclonal antibody FITC labled to CD102

Spezifität: Recognizes mouse CD102

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: COS cells transfected with mouse ICAM-2 cDNA

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu ICAM2

Target: ICAM2 (Intercellular Adhesion Molecule 2 (ICAM2))

Andere Bezeichnung: ICAM2

Hintergrund: Intercellular adhesion molecule 2, ICAM-2, CD102

Gen-ID: 15896