Rekombinanter Topoisomerase I Antikörper

Produktnummer ABIN7828628

Der Kaninchen Monoklonal anti-Topoisomerase I Antikörper wird verwendet zum Nachweis von Topoisomerase I in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, IF, IP und FACS.

Kurzübersicht für Rekombinanter Topoisomerase I Antikörper (ABIN7828628)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase I Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Klon: A475

Produktdetails anti-Topoisomerase I Antikörper

Verwendungszweck: Recombinant Topoisomerase I Monoclonal Antibody

Aufreinigung: Protein A purified

Immunogen: Recombinant human Topoisomerase I fragment

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000,IHC 1:100-1:500,IF 1:50,FCM 1:50-1:100,IP 1:25-1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Haltbarkeit: 12 months

Details zu Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Andere Bezeichnung: Topoisomerase I

Hintergrund: TOP,TOP1,TOPI,DNA topoisomerases play essential roles in many DNA metabolic processes including DNA repair. Topoisomerases can introduce DNA damage upon exposure to drugs that stabilize the covalent protein-DNA intermediate of the topoisomerase reaction. Lesions in DNA are also able to trap topoisomerase-DNA intermediates. DNA topoisomerase I (Top1) catalyzes the relaxation of supercoiled DNA by a mechanism of transient DNA strand cleavage characterized by the formation of a phosphotyrosyl bond between the DNA end and active site tyrosine. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molekulargewicht:

Calculated MW: 91 kDa

Observed MW: 108 kDa The actual band is not consistent with the expectation.

UniProt: P11387

Pathways: Caspase Kaskade in der Apoptose, Stem Cell Maintenance