Rekombinanter RNF40 Antikörper

Produktnummer ABIN7828533

Der Kaninchen Monoklonal anti-RNF40 Antikörper (Klon A775) (ABIN7828533) detektiert spezifisch RNF40 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Rekombinanter RNF40 Antikörper (ABIN7828533)

Target: RNF40 (Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser RNF40 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: A775

Produktdetails anti-RNF40 Antikörper

Verwendungszweck: Recombinant RNF40 Monoclonal Antibody

Aufreinigung: Protein A purified

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: WB 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Haltbarkeit: 12 months

Details zu RNF40

Target: RNF40 (Ring Finger Protein 40, E3 Ubiquitin Protein Ligase (RNF40))

Andere Bezeichnung: RNF40

Hintergrund: RBP,RNF,KIAA,RNF40,BRE1B,RBP95,STARING,KIAA0661,In mammalian cells, the significance of histone H2B ubiquitination in chromatin epigenetics came from the identification of the budding yeast protein Bre1. Together with the ubiquitin-conjugating enzyme Rad6, Bre1 serves as the E3 ligase in the monoubiquitination of the yeast histone H2B within transcribed regions of chromatin. Subsequently, the mammalian orthologs of yeast Bre1, RNF20 and RNF40, were identified. These two proteins form a tight heterodimer that acts as the major E3 ligase responsible for histone H2B monoubiquitination at Lys120 in mammalian cells, a modification linked to RNA Pol II-dependent transcription elongation in undamaged cells. Researchers have shown that DNA double-strand breaks (DSBs) are also capable of inducing monoubiquitination of H2B. This process depends upon the recruitment to DSB sites, as well as ATM-dependent phosphorylation of the RNF20-RNF40 heterodimer, thus highlighting a role for this E3 ligase in DSB repair pathways. Indeed, investigators have shown that loss of RNF20-RNF40 function promotes replication stress and chromosomal instability, which may constitute an early step in malignant transformation that precedes cell invasion. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molekulargewicht:

Calculated MW: 114 kDa

Observed MW: 140 kDa The actual band is not consistent with the expectation.

UniProt: O75150