Feimin Antikörper

Produktnummer ABIN7822917

Hersteller Produkt- Nr.: 200-401-nh2

Der Kaninchen Polyklonal anti-Feimin Antikörper wird verwendet zum Nachweis von Feimin in Proben von Human und Maus. Er wurde validiert für ELISA, IP und WB.

Kurzübersicht für Feimin Antikörper (ABIN7822917)

Target: Feimin

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Immunoprecipitation (IP), Western Blotting (WB)

Produktdetails

Hersteller Produkt- Nr.: 200-401-nh2

Hersteller: Rockland

Verwendungszweck: Anti-Feimin (RABBIT) Antibody

Kreuzreaktivität (Details): A BLAST analysis of the sequence of the immunogen shows 100% identity with mouse, 97% bovine, and 94% human. This product reacts with mouse, and human Feimin isoform 1 and 2. Reactivity against homologues from other sources is not known.

Aufreinigung: Anti-Feimin Antibody is purified from monospecific antiserum by Protein A affinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant mouse Feimin protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Feimin (RABBIT) Antibody has been tested by ELISA, Western Blot, and Immunoprecipitation. The antibody detects both human and mouse feimin protein from overexpressing cell lysates (using HEK293T and E. coli). A band approximately 20.1kDa in size corresponding to C5orf24 is detected. Cell lines expressing feimin can be expected using appropriate tissues and lysates. Specific conditions for reactivity should be optimized by the end user.

Kommentare:

ELISA Dilution: 1:44,500-1:64,500
Western Blot Dilution: 40 ng/mL
Immunoprecipitation Dilution: 40 ng/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.18 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: Feimin

Hintergrund: Background: The Feimin protein, also known as UPF0461 protein or C5orf24, involves regulatory roles within the cell and its exact role is under investigation. Feimin is a newly discovered myokine that complements insulin by fine-tuning glucose levels and boosting exercise capacity, especially under conditions where insulin is less effective. Highly conserved Feimin functions in both glucose homeostasis and has a direct role in muscle thermogenesis. Feimin can be secreted by muscle and act as a myokine where it can affect tissues by engaging MERTK receptor thereby activating AKT. Cellular Feimin (cFeimin) functions as a transcription regulator by interacting with FOXC2 protein, where it helps to enhance exercise performance by reducing thermogenesis.
Synonyms: Chromosome 5 Open Reading Frame 24, UPF0461 Protein C5orf24, FLJ37562, Feimin

Gen-ID: 134553

NCBI Accession: NP_001129058.1

UniProt: Q80X32