Rekombinanter CD370 & PD-L1 Antikörper

Produktnummer ABIN7795542

Der Maus Bispecific anti-CD370 & PD-L1 Antikörper wird verwendet zum Nachweis von CD370 & PD-L1 in Proben von Maus. Er wurde validiert für ELISA, FACS, ICC, IHC, in vivo und WB.

Kurzübersicht für Rekombinanter CD370 & PD-L1 Antikörper (ABIN7795542)

Target: CD370 & PD-L1

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Maus

Klonalität: Bispecific

Applikation: ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), In vivo Studies (in vivo), Western Blotting (WB)

Güteklasse: In vivo Grade

Klon: 10B4-10F-9G2-1

Produktdetails

Expressionssystem: Mammalian cells

Verwendungszweck: In Vivo Grade Recombinant Anti-mouse CD370 / PD-L1 Bispecific Antibody, Mouse IgG2c LALAPG Kappa (Clone 10B4 / 10F.9G2.1)

Produktmerkmale: The rat anti-mouse CD370 (Clec9A or DNGR-1) monoclonal antibody 10B4 (rat IgG2a kappa) reacts with the mouse CD370 protein. CLEC9A (C-type lectin domain family 9 member A), a transmembrane glycoprotein with a single extracellular C-type lectin domain (CTLD), plays a role in the immune response. As an activation receptor, CLEC9A is expressed on myeloid lineage cells, helps process material from dead cells, recognizes filamentous actin when cell membranes are damaged, and helps present antigens to cytotoxic T cells. CLEC9A has an intracellular cytoplasmic tail with potential signaling motifs. CLEC9A is expressed on a subset of BDCA3+ dendritic cells and CD16- monocytes in humans, and is expressed on CD8+ conventional DCs and plasmacytoid DCs in mice. The rat anti-mouse PD-L1 monoclonal antibody 10F.9G2 (rat IgG2b kappa) reacts with the mouse PD-L1 protein (programmed death ligand-1, B7-H1 or CD274), a member of the B7 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD-1 and anti-mouse PD-L1 antibodies which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The 10F.9G2 monoclonal antibody blocks the binding of the mouse PD-L1 protein to the mouse PD-1 protein. It is under investigation how the recombinant anti-mouse CD370 / PD-L1 bispecific antibodies (10B4 / 10F.9G2) behave different from the individual monoclonal antibodies and their combination. Our recombinant anti-mouse CD370 / PD-L1 bispecific antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD370 antibody (hybridoma clone name or number: 10B4) and the rat anti-mouse PD-L1 antibody (hybridoma clone name or number: 10F.9G2).

Aufreinigung: Protein A or G affinity column

Reinheit: >95 % by reducing SDS-PAGE

Endotoxin-Niveau: < 1 EU per 1 mg of the protein by the LAL method

Isotyp: IgG2c, L234A, L235A, P329G, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: > 3 mg/mL

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.

Antigendetails

Target: CD370 & PD-L1

Sub Type: Bispecific