EpCAM Antikörper

Produktnummer ABIN7674796

Der Maus Monoklonal Anti-EpCAM-Antikörper wurde für WB, FACS, IHC (p), ICC, IHC (fro) und D validiert. Er ist geeignet, EpCAM in Proben von Human zu detektieren.

Kurzübersicht für EpCAM Antikörper (ABIN7674796)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EpCAM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Detection (D)

Klon: HEA125

Produktdetails anti-EpCAM Antikörper

Verwendungszweck: The antibody HEA125 is directed against human EpCAM.

Spezifität: The antibody HEA125 is directed against human EpCAM.

Kreuzreaktivität (Details): The monoclonal antibody HEA125 shows a broad reactivity pattern with most human epithelial cells and epithelium derived tumours. It doesn't react with epidermal keratinocytes, parietal cells, hepatocytes, thymus cortical epithelium, myoepithelia, and non-epithelial tissues. HEA125 reacts intensely with all carcinomas derived from the intestinal tract, stomach, pancreas, liver, lung, breast, ovary, thyroid gland, kidney, bladder, prostate and metastases thereof. Stratified squamous epithelia usually stain less intensely then adenocarcinoma. The antibody is suitable for differentiation between carcinoma and non-carcinoma. Keratinizing areas of a tumour mass usually remain unstained as sarcoma, lymphoma, melanoma and neurogenic tumours do.

Produktmerkmale: Ep-CAM is a 34 kD glycoprotein and can be detected at the basolateral membrane of the majority of epithelial tissues. It is intricately linked with the cadherin-catenin complex and hence the fundamental WNT pathway responsible for intracellular signalling and polarity. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule. Of particular interest, Ep-CAM appears to be overexpressed at the cell surface of the majority of human epithelial carcinomas, including colorectal, breast, prostate, head and neck, and hepatic carcinomas. The antigen is therefore being used as a target for immunotherapy of human carcinomas. Also, Ep-CAM monoclonal antibodies are now extensively used for the detection and enrichment of circulating tumor cells in peripheral blood. Formation of Ep- CAM-mediated adhesions has a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression is suggested to be associated with enhanced epithelial proliferation.

Aufreinigung: ProtG affinity chromatography

Immunogen: HEA125 is a mouse monoclonal IgG1 antibody derived by fusion of Ag8.653 mouse myeloma cells with splenocytes from a BALB/c mouse cells immunized with the HT-29 colon carcinoma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: The HEA125 antibody is suitable for the detection of human EpCAM by Western blotting (under reducing and non-reducing conditions), flow cytometry and immunocytochemistry. The antibody is also suitable for the detection of human EpCAM by immunohistochemistry on frozen and paraffin embedded tissues. For the latter application, pre-treatment with pronase 0.1 % or unmasking fluid C (Art. No. DE000) is recommended. Optimal antibody dilutions for the different applications should be determined by titration. For immmunohistochemistry a dilution of 1:100 to 1:500 is recommended. The monoclonal antibody HEA125 shows a broad reactivity pattern with most human epithelial cells and epithelium derived tumours. It doesn't react with epidermal keratinocytes, parietal cells, hepatocytes, thymus cortical epithelium, myoepithelia, and non-epithelial tissues.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Each vial contains 100 μg 1 mg/mL purified monoclonal antibody in PBS containing 0.09 % sodium azide.

Konzentration: 1 mg/mL

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The antibody may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Details zu EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung: EpCAM

UniProt: P16422