Rekombinanter Vimentin Antikörper

Produktnummer ABIN7674794

Dieses Anti-Vimentin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Vimentin in WB, IHC, ICC, IHC (fro) und D. Geeignet für Maus.

Kurzübersicht für Rekombinanter Vimentin Antikörper (ABIN7674794)

Target: Vimentin (VIM)

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Detection (D)

Klon: RV204

Produktdetails anti-Vimentin Antikörper

Verwendungszweck: Recombinant mouse anti vimentin

Spezifität: Human and mouse vimentin

Produktmerkmale: Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

Aufreinigung: ProtA purified

Immunogen: This clone RV204 is a recombinant mouse anti vimentin monoclonal antibody produced in mammalian HEK293 cells. Clone RV204 is produced by cloning the antibody sequence of the mouse anti vimentin hybridoma RV202, which was derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: This clone RV204 is suitable for immunoblotting, immunocytochemistry, and immunohistochemistry on frozen and paraffin-embedd tissue sections. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with fluorochrome conjugated secondary antibodies or with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Each vial contains 100 µL 1 mg/mL ProtA purified recombinant monoclonal antibody in PBS containing 0.09 % sodium azide. The purity of the antibody is >98 % as determined by SDS-Polyacrylamide gel electrophoresis. The endotoxin level is < 1EU/mg as determined by the LAL chromogenic endotoxin assay.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: vimentin

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose