Elastin Antikörper (AA 681-786)

Produktnummer ABIN734003

Der Kaninchen Polyklonal Anti-Elastin-Antikörper wurde für WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) und FACS validiert und wurde unabhängig validiert. Er ist geeignet, Elastin in Proben von Human, Maus und Ratte zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für Elastin Antikörper (AA 681-786) (ABIN734003)

Target: Elastin (ELN)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Elastin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS)

Produktdetails anti-Elastin Antikörper

Bindungsspezifität: AA 681-786

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Elastin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IHC)

Validierung #028751 (Immunohistochemistry)

by: Beth Israel Deaconess Medical Center Confocal Imaging Core

No.: #028751

Datum: 25.09.2013

Chargennummer: 120306

Validierte Anwendung: Immunohistochemistry

Positivkontrolle: Mouse heart tissue

Negativkontrolle: Mouse adipose tissue

Bewertung: Signal was detected in positive control tissue and not detected in negative control tissue.

Validierungsbilder

Figure 1: Immunostaining of Elastin antibody. Panel 1: Staining of mouse heart tissue stained with anti-Elastin antibody (brown). Panel 2: Staining of mouse adipose tissue with anti-Elastin antibody. Panel 3: Staining of mouse heart tissue with isotype control antibody. Panel 4: Staining of mouse heart tissue with secondary antibody only. All panels are counterstained with hematoxylin (blue).

Protokoll

Primärantikörper:

• Antibody: Elastin (ELN) (C-Term) antibody
• Catalog number: ABIN734003
• Lot number: 120306

Sekundärantikörper:

• Antibody: Donkey anti-Rabbit IgG Antibody (Biotinylated)

Full Protocol:

• Immunohistochemistry was performed manually.
• Sections were deparaffinized and rehydrated.
• Sections were heated to 98°C for 10 min in 10 mM citrate buffer pH 6.0 for antigen retrieval.
• Sections were incubated in 3% hydrogen peroxide for 10 min at room temperature to block endogenous peroxidase.
• Sections were washed x 3 in Tris buffered saline (TBS).
• Sections were blocked in 5% normal donkey serum for 60 min at room temperature.
• Sections were incubated with primary antibody diluted 1:1000 in 5% normal donkey serum in TBS. Incubated at 4°C overnight.
• Sections were washed x 3 in Tris buffered saline.
• Sections were incubated with secondary antibody diluted 1:400 in 5% normal donkey serum in TBS. Incubated for 60 min at room temperature.
• Sections were washed x 3 in Tris buffered saline.
• Sections were incubated with Vectastain ABC kit (Vector Lab PK6100) to enhance signal.
• Sections were incubated with DAB chromogenic substrate (Vector Lab SK-4105) for 60 seconds at room temperature.
• Sections were washed x 3 in Distilled Water.
• Sections were counterstained with hematoxylin for 1 min.
• Sections were washed x 1 in Distilled Water.
• Sections were dehydrated, mounted and photographed under a Zeiss AxioImager M1 light microscope.

Anmerkungen: None

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-Elastin Antikörper (ABIN734003)

Hasegawa, Sato, Shirai, Watanabe, Yamamoto, Watanabe, Nohtomi, Hirano, Watanabe: "Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivo." in: PLoS ONE, Vol. 9, Issue 12, pp. e110866, (2014) (PubMed).

Konii, Sato, Kikuchi, Okiyama, Watanabe, Hasegawa, Yamamoto, Itoh, Hirano, Watanabe: "Stimulatory effects of cardiotrophin 1 on atherosclerosis." in: Hypertension, Vol. 62, Issue 5, pp. 942-50, (2013) (PubMed).

Guo, Li, Dong, Chen, Deng, Wang, Ying: "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." in: Colloids and surfaces. B, Biointerfaces, Vol. 96, pp. 29-36, (2012) (PubMed).

Ikeda, Yada, Takase: "[Syntheses of 1,3,4-thiadiazole derivatives]." in: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, Vol. 90, Issue 1, pp. 95-8, (1970) (PubMed).

Details zu Elastin

Target: Elastin (ELN)

Andere Bezeichnung: Elastin

Hintergrund:

Synonyms: WS, WBS, SVAS, Elastin, Tropoelastin, ELN

Background: Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).

Gen-ID: 2006

UniProt: P15502

Pathways: Regulation of Actin Filament Polymerization