MBP Tag Antikörper

Produktnummer ABIN7673460

Der Maus Monoklonal Anti--Antikörper wurde für WB, ELISA und IP validiert. Er ist geeignet, in Proben von zu detektieren.

Kurzübersicht für MBP Tag Antikörper (ABIN7673460)

Target: MBP Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Klon: 0D8

Produktdetails

Verwendungszweck: MBP tag antibody

Aufreinigung: Protein A+G purification

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: MBP-Tag

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: WB: 1:1000-1:8000, IP: 1:5000-1:50000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Haltbarkeit: 12 months

Antigendetails

Target: MBP Tag

Substanzklasse: Tag

Hintergrund:

Synonyms: Maltose Binding Protein|MBP|MBP Tag

Background: Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin.

Molekulargewicht: 40 kDa