WT1 Antikörper (AA 1-181) (CF®740)

Produktnummer ABIN7662044

Der Maus Monoklonal Anti-WT1-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, WT1 in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für WT1 Antikörper (AA 1-181) (CF®740) (ABIN7662044)

Target: WT1 (Wilms Tumor 1 (WT1))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser WT1 Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: WT1-857 6F-H2

Produktdetails anti-WT1 Antikörper (CF®740)

Bindungsspezifität: AA 1-181

Verwendungszweck: WT1 (Wilm's Tumor 1)(WT1/857 + 6F-H2), CF740 conjugate

Produktmerkmale: Recognizes a 47-55 kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors.WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full length human WT1 protein (WT1/857), Recombinant fragment aa1-181 of human WT1 (6F-H2)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL

Kommentare:

Positive Control: K562 cells. Wilm s Tumor, mesothelioma or fetal kidney.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Andere Bezeichnung: Wilms Tumor 1

Hintergrund:

Synonyms: WT1, AWT1, FWT1, GUD, NPHS4, WAGR, Wilms tumor 1

Gene Symbol: WT1

Molekulargewicht: 47-55 kDa

Gen-ID: 7490

UniProt: P19544

Pathways: Tube Formation