Vimentin Antikörper (AA 2-466) (CF®594)

Produktnummer ABIN7661942

Der Maus Monoklonal anti-Vimentin Antikörper wird verwendet zum Nachweis von Vimentin in Proben von Human. Er wurde validiert für WB, IHC und IHC (fp).

Kurzübersicht für Vimentin Antikörper (AA 2-466) (CF®594) (ABIN7661942)

Target: Vimentin (VIM)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist konjugiert mit CF®594

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: VIM-3736

Produktdetails anti-Vimentin Antikörper (CF®594)

Bindungsspezifität: AA 2-466

Verwendungszweck: Vimentin (Mesenchymal Cell Marker)(VIM/3736), CF594 conjugate

Produktmerkmale: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e. g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A recombinant fragment (around aa 2-466) of human Vimentin protein

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: U87, A375, Raji, HEP2 cells. Sarcomas or Melanomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: Vimentin

Hintergrund:

Synonyms: VIM

Gene Symbol: VIM

Molekulargewicht: 57-60 kDa

Gen-ID: 7431

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose