VEGF Antikörper

Produktnummer ABIN7661850

Dieses Anti-VEGF-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von VEGF in WB, IHC, IHC (fp) und Func. Geeignet für Human.

Kurzübersicht für VEGF Antikörper (ABIN7661850)

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VEGF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Functional Studies (Func)

Klon: VG76e

Produktdetails anti-VEGF Antikörper

Verwendungszweck: VEGF (Vascular Endothelial Growth Factor)(VG76e)

Produktmerkmale: This MAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet-derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human VEGF189 recombinant protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: ELISA: For coating, order Ab without BSA, Immunofluorescence: 1-2 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Kommentare:

Positive Control: Tumor cells in astrocytomas, breast or ovarian carcinomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Andere Bezeichnung: VEGF

Hintergrund:

Synonyms: MVCD1, Vascular Endothelial Growth Factor A (VEGF-A), Vascular Permeability Factor (VPF), VEGFA

Gene Symbol: VEGFA

Tissue Expression: Endothelial cells

Molekulargewicht: 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing)

Gen-ID: 7422

UniProt: P15692