Transgelin Antikörper (CF®740)

Produktnummer ABIN7661060

Der Maus Monoklonal Anti-Transgelin-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, Transgelin in Proben von Human, Maus, Kaninchen, Rind (Kuh) und Schwein zu detektieren.

Kurzübersicht für Transgelin Antikörper (CF®740) (ABIN7661060)

Target: Transgelin (TAGLN)

Reaktivität: Human, Maus, Kaninchen, Rind (Kuh), Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Transgelin Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: TAGLN-247

Produktdetails anti-Transgelin Antikörper (CF®740)

Verwendungszweck: Transgelin / SM22-alpha(TAGLN/247), CF740 conjugate

Produktmerkmale: This MAb recognizes a 22 kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is downregulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human transgelin (TAGLN) protein.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL

Kommentare:

Positive Control: U-2 OS, Hs68 or WI 38 cells. Colon carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Transgelin

Target: Transgelin (TAGLN)

Andere Bezeichnung: SM22-Alpha

Hintergrund:

Synonyms: 2 kDa actin-binding protein, Human 22 kDa smooth muscle protein, Protein WS3-10, SMCC, Smooth muscle protein 22-alpha (SM22-alpha), TAGLN, TAGLN1, transgelin, Transgelin variant 2, WS3-10

Gene Symbol: TAGLN

Tissue Expression: Smooth muscle

Molekulargewicht: 22 kDa

Gen-ID: 6876

UniProt: Q01995