Transferrin Antikörper

Produktnummer ABIN7661012

Der Maus Monoklonal Anti-Transferrin Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, Transferrin in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für Transferrin Antikörper (ABIN7661012)

Target: Transferrin (TF)

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Transferrin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: A78-G-A7

Produktdetails anti-Transferrin Antikörper

Verwendungszweck: Thomsen-Friedenreich Antigen / CD176 (Pan Carcinoma Marker)(A78-G/A7)

Produktmerkmale: This antibody recognizes a disaccharide epitope, Gal 1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF , including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. This MAb is capable to agglutinate desialylated red blood cells. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide. This antibody is available purified, with BSA and azide (0.2 mg/mL) or purified, BSA-free and azide-free (1 mg/mL).

Immunogen: Neuraminidase-treated human red blood cells

Isotyp: IgM, kappa

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (formalin-fixed): 0.5-1.0 μg/mL for 30 minutes at RT. Immunofluorescence: 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: KG1 cells. Human colorectal carcinoma tissues.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Transferrin

Target: Transferrin (TF)

Andere Bezeichnung: CD176

Hintergrund:

Synonyms: T-F Antigen, TF Antigen, Asialoglycophorin, pan-carcinoma marker, CD176

Gene Symbol: Not Applicable

Molekulargewicht: Multiple

Pathways: Transition Metal Ion Homeostasis