TIMP1 Antikörper (AA 72-82) (CF®640R)

Produktnummer ABIN7660836

Dieses Anti-TIMP1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von TIMP1 in WB, IHC und IHC (fp). Geeignet für Human.

Kurzübersicht für TIMP1 Antikörper (AA 72-82) (CF®640R) (ABIN7660836)

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TIMP1 Antikörper ist konjugiert mit CF®640R

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: 2A5

Produktdetails anti-TIMP1 Antikörper (CF®640R)

Bindungsspezifität: AA 72-82

Verwendungszweck: TIMP1 (Marker of Lymph Node Metastasis)(2A5), CF640R conjugate

Produktmerkmale: TIMP-1, TIMP-2, TIMP-3 and TIMP-4 (for tissue inhibitor of metalloproteinases -1, -2, -3 and -4) complex with metalloproteinases such as collagenases, gelatinases and stromelysins, resulting in irreversible inactivation of the metalloproteinase. TIMP-1 is identical to EPA (erythroid-potentiation activity). PTH has been shown to be a regulator of TIMP-2 in osteoblastic cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes. TIMP-4 is most highly expressed in heart tissues. Studies have demonstrated that TIMP1 is useful as a biomarker for early detection of colorectal cancer, outperforming CEA. Additionally, TIMP1 studies have demonstrated its role in CRC tumorigenesis, as well as observing its overexpression in metastatic lymph nodes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide (aa 72-82) of human TIMP1 protein (FQALGDAADIR)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: ELISA: Use Ab at 2-4 μg/mL for coating, Order Ab without BSA, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Kommentare:

Positive Control: 293 cells. Colon Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu TIMP1

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Andere Bezeichnung: TIMP1

Hintergrund:

Synonyms: Collagenase inhibitor, EPO, Erythroid-Potentiating Activity (EPA), Fibroblast collagenase inhibitor, Human Collagenase Inhibitor (HCI), Metalloproteinase inhibitor 1, Tissue Inhibitor of Metalloproteinase 1 (TIMP1)

Gene Symbol: TIMP1

Molekulargewicht: 23 kDa

Gen-ID: 7076

UniProt: P01033