TYMS Antikörper (AA 60-174)

Produktnummer ABIN7660741

Dieses Maus Monoklonal-Antikörper erkennt spezifisch TYMS in IF, FACS, IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für TYMS Antikörper (AA 60-174) (ABIN7660741)

Target: TYMS (Thymidylate Synthetase (TYMS))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TYMS Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: TYMS-1884

Produktdetails anti-TYMS Antikörper

Bindungsspezifität: AA 60-174

Verwendungszweck: Thymidylate Synthase (5-FU Resistance Marker)(TYMS/1884)

Produktmerkmale: This antibody recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human thymidylate synthase protein fragment (around aa 60-174)

Isotyp: IgG2c, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: MOLT4, Ramos, 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Andere Bezeichnung: Thymidylate Synthase

Substanzklasse: Viral Protein

Hintergrund:

Synonyms: dTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase

Gene Symbol: TYMS

Molekulargewicht: 36 kDa

Gen-ID: 7298

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases