Rekombinanter TYMS Antikörper (AA 60-174) (CF®647)

Produktnummer ABIN7660725

Dieses Anti-TYMS-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von TYMS in IF und FACS. Geeignet für Human.

Kurzübersicht für Rekombinanter TYMS Antikörper (AA 60-174) (CF®647) (ABIN7660725)

Target: TYMS (Thymidylate Synthetase (TYMS))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TYMS Antikörper ist konjugiert mit CF®647

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: RTYMS-1884

Produktdetails anti-TYMS Antikörper (CF®647)

Bindungsspezifität: AA 60-174

Verwendungszweck: Thymidylate Synthase (5-FU Resistance Marker)(rTYMS/1884), CF647 conjugate

Produktmerkmale: Recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2. 1. 1. 45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5, 10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human thymidylate synthase protein fragment (around aa 60-174)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: ELISA: For coating, order antibody without BSA, Flow cytometry: 0.5-1 μg/million cells, Immunofluorescence: 0.5-1 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Kommentare:

Positive Control: MOLT4, Ramos, 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Andere Bezeichnung: Thymidylate Synthase

Substanzklasse: Viral Protein

Hintergrund:

Synonyms: dTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase

Gene Symbol: TYMS

Molekulargewicht: 36 kDa

Gen-ID: 7298

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases