Testosterone Antikörper (CF®740)

Produktnummer ABIN7660684

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Testosterone in IHC, RIA und IHC (fp). Er zeigt eine Reaktivität gegenüber Diverse Spezies.

Kurzübersicht für Testosterone Antikörper (CF®740) (ABIN7660684)

Target: Testosterone

Reaktivität: Diverse Spezies

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Testosterone Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Radioimmunoassay (RIA), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: 4E1G2

Produktdetails anti-Testosterone Antikörper (CF®740)

Verwendungszweck: Testosterone(4E1G2), CF740 conjugate

Produktmerkmale: This MAb is highly specific to testosterone. Its affinity constant for testosterone is ~1010 M-1. In competitive binding immunoassay, it reacts with testosterone 100%, 11-beta-hydroxy testosterone 3.3%, 17-alpha-methyl testosterone <0.1%, 5-alpha DHT 0.8%, estradiol <0.1% and progesterone <0.1%. Testosterone, a steroid hormone from the androgen group, is derived from cholesterol. Testosterone is primarily secreted in the testes, but the ovaries and adrenal glands also produce smaller amounts. It is the principal male sex hormone and is responsible for the maturation of sex organs and the development of male secondary sex characteristics. The hormone is also involved in the growth of muscle mass and increases bone density and maturation. It functions by activating the androgen receptor and converting to estradiol and activation of estrogen receptors. Testosterone has been employed therapeutically to treat many conditions including infertility, lack of libido, osteoporosis, height growth and bone marrow stimulation. Anti-testosterone antibody may also prove useful in identification of testicular tumors arising from interstitial cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Testosterone 3 CMO conjugated to BSA

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Immunohistology formalin-paraffin 0.25-0.5 μg/mL. ELISA For coating, order Ab without BSA. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Interstitial or Leydid cells in testis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Testosterone

Target: Testosterone

Substanzklasse: Hormone

Hintergrund:

Synonyms: 17i²-Hydroxy-3-oxo-4-androstene, 17i²-Hydroxy-4-androsten-3-one, 4-Androsten-17i²-ol-3-one, trans-Testosterone

Tissue Expression: Testis

Molekulargewicht: 288.42Da