Rekombinanter Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) Antikörper (CF®405S)

Produktnummer ABIN7660167

Dieses Anti--Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von in WB. Geeignet für Human.

Kurzübersicht für Rekombinanter Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) Antikörper (CF®405S) (ABIN7660167)

Target: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: CF®405S

Applikation: Western Blotting (WB)

Klon: RSPTBN2-1778

Produktdetails

Bindungsspezifität: AA 356-475

Verwendungszweck: Spectrin beta III (SPTBN2)(rSPTBN2/1778), CF405S conjugate

Produktmerkmale: Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II) four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin and spectrin are present in erythrocytes, whereas spectrin II (also designated fodrin ) and spectrin I (also designated fodrin ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin III is highly homologous to both spectrin I and spectrin II. Spectrin III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa356-475) of human SPTBN2 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: ELISA: Use Ab at 2-4 μg/mL for coating, Order Ab without BSA, Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Kommentare:

Positive Control: PANC-1, HeLa, PC-3 cells. Human brain, pancreas or liver tissues (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Antigendetails

Target: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Andere Bezeichnung: Spectrin Beta-III

Hintergrund:

Synonyms: Beta III spectrin, SCA5, Spectrin beta chain brain 2, Spectrin beta non-erythrocytic 2, Spectrin non-erythroid beta chain 2, Spinocerebellar ataxia 5, SPTBN2

Gene Symbol: SPTBN2

Molekulargewicht: 246 kDa

Gen-ID: 6712

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis