Rekombinanter CD43 Antikörper

Produktnummer ABIN7659934

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch CD43 in FACS, WB, IHC, IF und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter CD43 Antikörper (ABIN7659934)

Target: CD43 (SPN) (Sialophorin (SPN))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD43 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: SPN-2049R

Produktdetails anti-CD43 Antikörper

Verwendungszweck: CD43 (T-Cell Marker) (SPN/2049R)

Produktmerkmale: This antibody recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43. From 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CD43 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Paracortex in a tonsil or a reactive lymph node

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Andere Bezeichnung: CD43

Hintergrund:

Synonyms: Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN

Gene Symbol: SPN

Tissue Expression: Lymphocytes

Molekulargewicht: 95, 115, or 135 kDa

Gen-ID: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity