L-Selectin Antikörper (Biotin)

Produktnummer ABIN7659758

Der Maus Monoklonal Anti-L-Selectin-Antikörper wurde für validiert. Er ist geeignet, L-Selectin in Proben von Human zu detektieren.

Kurzübersicht für L-Selectin Antikörper (Biotin) (ABIN7659758)

Target: L-Selectin (SELL) (Selectin L (SELL))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser L-Selectin Antikörper ist konjugiert mit Biotin

Applikation: Bitte anfragen

Klon: CD62L-1588

Produktdetails anti-L-Selectin Antikörper (Biotin)

Verwendungszweck: CD62L (L-Selectin)(CD62L/1588), Biotin conjugate

Produktmerkmale: Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: MAb raised against supernatant from phorbol myristic acid activated human peripheral blood leukocytes.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: HPBL or Jurkat whole cell lysate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu L-Selectin

Target: L-Selectin (SELL) (Selectin L (SELL))

Andere Bezeichnung: CD62L

Hintergrund:

Synonyms: A.11, AI528707, CD62 antigen ligand, CD62L, gp90-MEL, IgA nephropathy, susceptibility to, included, L Selectin, L-selectin, LAM1, LECAM1, LEU8, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lnhr, LSEL, Ly-22, LYAM1, Lymph node homing receptor, Lymphocyte antigen 22, Lymphocyte surface MEL-14 antigen, Pln homing receptor, PLNHR, SELL, TQ1

Gene Symbol: SELL

Tissue Expression: Eosinophils|Lymphocytes|Monocytes/macrophages|Neutrophils

Molekulargewicht: 65-75 kDa

Gen-ID: 6402

UniProt: P14151