s100a4 Antikörper (CF®640R)

Produktnummer ABIN7659697

Dieses Maus Monoklonal-Antikörper erkennt spezifisch s100a4 in IHC, IF, FACS und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für s100a4 Antikörper (CF®640R) (ABIN7659697)

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser s100a4 Antikörper ist konjugiert mit CF®640R

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: CPTC-S100A4-3

Produktdetails anti-s100a4 Antikörper (CF®640R)

Verwendungszweck: S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis)(CPTC-S100A4-3), CF640R conjugate

Produktmerkmale: S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100A4 has been implicated in the progression and prognosis of several forms of human cancer, e. g. breast, colorectal, gastric, pancreatic and bladder cancer, SCLC and oesophageal squamous cell carcinoma, among others. Poor prognosis associated with high S100A4 expression is accompanied by clear signs of disease progression, e. g. high histological and clinical grades and involvement of lymph nodes. Also indicative of poor prognosis is high S100A4 expression coupled with reduced E-cadherin expression in pancreatic, oral squamous cell carcinoma and in melanoma. S100A4 expression is inversely related with expression of metastasis suppressor nm23 and with prognosis of breast cancer. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human S100A4 full length protein

Isotyp: IgG2c, kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1-2 μg/million cells, Immunofluorescence: 1-2 μg/mL, Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Kommentare:

Positive Control: T98G, HeLa, A549 or A375 cells. Placenta, small intestine.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Andere Bezeichnung: Calvasculin

Hintergrund:

Synonyms: S100 calcium-binding protein A4, Calvasculin, CAPL, Fibroblast specific protein 1 (FSP1), Leukemia multidrug resistance associated protein, Malignant transformation suppression 1 (MTS1), Metastasin, Placental calcium-binding protein

Gene Symbol: S100A4

Molekulargewicht: 12 kDa

Gen-ID: 6275

UniProt: P26447

Pathways: S100 Proteine