RNA Polymerase II Antikörper (pSer5) (CF®594)

Produktnummer ABIN7659657

Dieses Anti-RNA Polymerase II-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von RNA Polymerase II in WB, IHC (fp) und IHC. Geeignet für Human, Maus und Ratte.

Kurzübersicht für RNA Polymerase II Antikörper (pSer5) (CF®594) (ABIN7659657)

Target: RNA Polymerase II

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RNA Polymerase II Antikörper ist konjugiert mit CF®594

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunohistochemistry (IHC)

Klon: CTD4H8

Produktdetails anti-RNA Polymerase II Antikörper (CF®594)

Bindungsspezifität: pSer5

Verwendungszweck: RNA Polymerase II(CTD4H8), CF594 conjugate

Produktmerkmale: RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1, 840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: HAP1, K562, PC3, HePG2, NIH3T3 cells. Human testis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu RNA Polymerase II

Target: RNA Polymerase II

Hintergrund:

Synonyms: DNA-directed RNA polymerase II largest subunit RNA polymerase II 220kd subunit, DNA-directed RNA polymerase II subunit RPB1, hRPB220, hsRPB1, POLR2, Polr2a, POLRA, Polymerase (RNA) II (DNA directed) polypeptide A 220 kDa, RNA polymerase II subunit B1, RPBh1, RpIILS, RPO2, RPOL2

Gene Symbol: POLR2A

Molekulargewicht: 192-253 kDa

Gen-ID: 5430

UniProt: P24928