Rekombinanter PSAP Antikörper (CF®647)
Kurzübersicht für Rekombinanter PSAP Antikörper (CF®647) (ABIN7659219)
Target
Antikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
-
-
Verwendungszweck
- Prostate Specific Acid Phosphatase (PSAP) (rACPP/1338), CF647 conjugate
-
Produktmerkmale
- This antibody recognizes a protein of 52 kDa, identified as prostate specific acid phosphatase (PSAP). This enzyme catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is synthesized under androgen regulation and is secreted by the epithelial cells of the prostate gland. PSAP is found in non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no staining in granulocytes, osteoclasts, parietal cells of the stomach, liver cells, renal cell or breast carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
-
Immunogen
- Recombinant full-length human ACPP protein
-
Isotyp
- IgG1, kappa
-
-
-
-
Applikationshinweise
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1.0 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
-
Kommentare
-
Positive Control: PC12 cells or normal prostate or prostate carcinoma
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Liquid
-
Konzentration
- 0.1 mg/mL
-
Buffer
- PBS, 0.1 % BSA, 0.05 % azide
-
Konservierungsmittel
- Sodium azide
-
Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handhabung
- Protect from light
-
Lagerung
- 4 °C
-
Informationen zur Lagerung
-
Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
-
Haltbarkeit
- 24 months
-
-
- PSAP (Prostate Specific Acid Phosphatase (PSAP))
-
Andere Bezeichnung
- Prostate-Specific Acid Phosphatase
-
Hintergrund
-
Synonyms: 5'-nucleotidase (5'-NT), Acid phosphatase prostate, ACP3, Ecto-5'-nucleotidase, Prostatic acid phosphatase (PAP), Prostatic acid phosphatase, Thiamine monophosphatase (TMPase)
Gene Symbol: ACPP
Tissue Expression: Prostate
-
Molekulargewicht
- 52 kDa
-
Gen-ID
- 55
-
UniProt
- P15309
Target
-