Plakophilin 1 Antikörper (N-Term) (CF®740)

Produktnummer ABIN7658623

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Plakophilin 1 in WB. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für Plakophilin 1 Antikörper (N-Term) (CF®740) (ABIN7658623)

Target: Plakophilin 1 (PKP1)

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Plakophilin 1 Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB)

Klon: 10B2

Produktdetails anti-Plakophilin 1 Antikörper (CF®740)

Bindungsspezifität: N-Term

Verwendungszweck: Plakophilin-1 (PKP1) (10B2), CF740 conjugate

Produktmerkmale: This antibody recognizes a protein of 75 kDa, identified as Plakophilin-1. Its epitope maps between aa 1-27 human Plakophilin-1. Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: N-terminus of human Plakophilin-1 protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HaCat Cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Plakophilin 1

Target: Plakophilin 1 (PKP1)

Andere Bezeichnung: Plakophilin-1

Hintergrund:

Synonyms: B6P, B, 6 protein, PKP1, Plakophilin-1 (ectodermal dysplasia/skin fragility syndrome)

Gene Symbol: PKP1

Tissue Expression: Cardiac

Molekulargewicht: 75 kDa

Gen-ID: 5317

UniProt: Q13835