pan Keratin Antikörper (acidic) (Biotin)

Produktnummer ABIN7658453

Der Maus Monoklonal Anti-pan Keratin-Antikörper wurde für validiert. Er ist geeignet, pan Keratin in Proben von Human, Ratte, Maus, Rind (Kuh), Affe, Hund, Kaninchen, Huhn und Schildkröte zu detektieren.

Kurzübersicht für pan Keratin Antikörper (acidic) (Biotin) (ABIN7658453)

Target: pan Keratin (panKRT)

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Affe, Hund, Kaninchen, Huhn, Schildkröte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser pan Keratin Antikörper ist konjugiert mit Biotin

Applikation: Bitte anfragen

Klon: AE-1

Produktdetails anti-pan Keratin Antikörper (Biotin)

Bindungsspezifität: acidic

Verwendungszweck: Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker)(AE-1), Biotin conjugate

Produktmerkmale: This MAb recognizes the 56. 5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e. g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human epidermal keratin

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: Skin or squamous cell carcinoma (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu pan Keratin

Target: pan Keratin (panKRT)

Andere Bezeichnung: Keratin

Hintergrund:

Synonyms: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Gene Symbol: KRT77

Tissue Expression: Epidermal cells|Epithelial cells

Molekulargewicht: 40-56.5 kDa

Gen-ID: 374454

UniProt: Q7Z794