pan Keratin Antikörper (High Molecular Weight) (CF®740)

Produktnummer ABIN7658426

Der Maus Monoklonal Anti-pan Keratin-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, pan Keratin in Proben von Human und Ratte zu detektieren.

Kurzübersicht für pan Keratin Antikörper (High Molecular Weight) (CF®740) (ABIN7658426)

Target: pan Keratin (panKRT)

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser pan Keratin Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: KRTH-1076

Produktdetails anti-pan Keratin Antikörper (CF®740)

Bindungsspezifität: High Molecular Weight

Verwendungszweck: Cytokeratin, HMW(KRTH/1076), CF740 conjugate

Produktmerkmale: This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human KRT76 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Western blotting 0.5-1 μg/mL. ELISA For coating, order Ab without BSA. Predicted to show broad species reactivity

Kommentare:

Positive Control: Skin, Adenocarcinomas

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu pan Keratin

Target: pan Keratin (panKRT)

Andere Bezeichnung: Keratin

Hintergrund:

Synonyms: KRT2B, KRT2P, HUMCYT2A, Keratin, Type II Cytoskeletal 2 Oral, K2P, K76, CK-2P, Keratin 2p, Keratin-76, Cytokeratin 2, Cytokeratin-2P, Type-II Keratin Kb9

Gene Symbol: KRT76

Tissue Expression: Epidermal cells|Epithelial cells

Molekulargewicht: 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8)

Gen-ID: 51350

UniProt: Q01546