pan Keratin Antikörper (CF®740)

Produktnummer ABIN7658424

Dieses Anti-pan Keratin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von pan Keratin in IHC und IHC (fp). Geeignet für Human, Ratte, Maus, Rind (Kuh), Affe, Schwein, Frosch, Meerschweinchen und Ziege.

Kurzübersicht für pan Keratin Antikörper (CF®740) (ABIN7658424)

Target: pan Keratin (panKRT)

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Affe, Schwein, Frosch, Meerschweinchen, Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser pan Keratin Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: KRT-457

Produktdetails anti-pan Keratin Antikörper (CF®740)

Verwendungszweck: Cytokeratin, multi(KRT/457), CF740 conjugate

Produktmerkmale: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Keratin-enriched preparation from cultured human epithelial cells

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 0.5-1.0 μg/mL. Western blotting 0.5-1.0 μg/mL

Kommentare:

Positive Control: A431 cells, Skin, Colon carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu pan Keratin

Target: pan Keratin (panKRT)

Andere Bezeichnung: Keratin, multi

Hintergrund:

Synonyms: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Gene Symbol: KRT4

Tissue Expression: Epidermal cells|Epithelial cells

Molekulargewicht: Multiple

Gen-ID: 3851