Rekombinanter pan Keratin Antikörper (pan) (CF®647)

Produktnummer ABIN7658405

Der Kaninchen Monoklonal Anti-pan Keratin-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, pan Keratin in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter pan Keratin Antikörper (pan) (CF®647) (ABIN7658405)

Target: pan Keratin (panKRT)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser pan Keratin Antikörper ist konjugiert mit CF®647

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: KRTH-1576R KRTL-1577R

Produktdetails anti-pan Keratin Antikörper (CF®647)

Bindungsspezifität: pan

Verwendungszweck: Cytokeratin, pan (Epithelial Marker) (KRTH/1576R + KRTL/1577R), CF647 conjugate

Produktmerkmale: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67 kDa (CK1); 64 kDa (CK3); 59 kDa (CK4); 58 kDa (CK5); 56 kDa (CK6); 52 kDa (CK8); 56.5 kDa (CK10); 50 kDa (CK14); 50 kDa (CK15); 48 kDa (CK16); 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. It is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It may be useful to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has high sensitivity in the recognition of epithelial cells and carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human KRT76 and KRT77 proteins

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Skin, Adeno- or Squamous carcinomas

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu pan Keratin

Target: pan Keratin (panKRT)

Andere Bezeichnung: Keratin

Hintergrund:

Synonyms: K1B; KRT1B; Keratin; type II cytoskeletal 1b; K77; CK-1B; Keratin 1B; Keratin-77; Cytokeratin-1B; Type-II Keratin Kb39

Gene Symbol: KRT77 & KRT76

Tissue Expression: Epidermal cells|Epithelial cells

Molekulargewicht: 40-67 kDa

Gen-ID: 374454, 51350

UniProt: Q7Z794, Q01546