pan Keratin Antikörper (acidic) (CF®647)

Produktnummer ABIN7658404

Dieses Maus Monoklonal-Antikörper erkennt spezifisch pan Keratin in . Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Rind (Kuh), Affe, Hund, Kaninchen, Huhn und Schildkröte.

Kurzübersicht für pan Keratin Antikörper (acidic) (CF®647) (ABIN7658404)

Target: pan Keratin (panKRT)

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Affe, Hund, Kaninchen, Huhn, Schildkröte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser pan Keratin Antikörper ist konjugiert mit CF®647

Applikation: Bitte anfragen

Klon: AE-1

Produktdetails anti-pan Keratin Antikörper (CF®647)

Bindungsspezifität: acidic

Verwendungszweck: Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker)(AE-1), CF647 conjugate

Produktmerkmale: This MAb recognizes the 56. 5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e. g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human epidermal keratin

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: Skin or squamous cell carcinoma (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu pan Keratin

Target: pan Keratin (panKRT)

Andere Bezeichnung: Keratin

Hintergrund:

Synonyms: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

Gene Symbol: KRT77

Tissue Expression: Epidermal cells|Epithelial cells

Molekulargewicht: 40-56.5 kDa

Gen-ID: 374454

UniProt: Q7Z794