P-Cadherin Antikörper (CF®740)

Produktnummer ABIN7658270

Der Maus Monoklonal Anti-P-Cadherin-Antikörper wurde für WB und IF validiert. Er ist geeignet, P-Cadherin in Proben von Human zu detektieren.

Kurzübersicht für P-Cadherin Antikörper (CF®740) (ABIN7658270)

Target: P-Cadherin (CDH3)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser P-Cadherin Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 12H6

Produktdetails anti-P-Cadherin Antikörper (CF®740)

Verwendungszweck: P-Cadherin (CDH3) (12H6), CF740 conjugate

Produktmerkmale: This antibody recognizes a protein of 116 kDa, identified as P-Cadherin-1 (CDH3). It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congenital hypotrichosis with juvenile macular dystrophy.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length P-cadherin fusion protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: A431 or PC-3 cells. Pancreas, Placenta or Prostate

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu P-Cadherin

Target: P-Cadherin (CDH3)

Andere Bezeichnung: P-Cadherin

Hintergrund:

Synonyms: Cadherin 3 type 1, Cadherin-3, Cadp, Calcium dependent adhesion protein placental, CDH3, CDHP, HJMD, P-cadherin (Placental), PCAD, Placental cadherin

Gene Symbol: CDH3

Tissue Expression: Epithelial cells

Molekulargewicht: 116 kDa

Gen-ID: 1001

UniProt: P22223