Occludin Antikörper (AA 282-415) (CF®568)

Produktnummer ABIN7658201

Der Maus Monoklonal Anti-Occludin-Antikörper wurde für WB, IHC und IHC (fp) validiert. Er ist geeignet, Occludin in Proben von Human zu detektieren.

Kurzübersicht für Occludin Antikörper (AA 282-415) (CF®568) (ABIN7658201)

Target: Occludin (OCLN)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Occludin Antikörper ist konjugiert mit CF®568

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: OCLN-2181

Produktdetails anti-Occludin Antikörper (CF®568)

Bindungsspezifität: AA 282-415

Verwendungszweck: Occludin (Marker of Early Blood Brain Barrier Damage)(OCLN/2181), CF568 conjugate

Produktmerkmale: Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue, in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Occludin is a tight junction protein that is a key structural component of the blood-brain barrier (BBB). Degradation of Occludin is frequently seen in ischemic stroke and contributes to BBB disruption. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human Occludin protein (around aa 282-415)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: HepG2 cells. Kidney.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Occludin

Target: Occludin (OCLN)

Andere Bezeichnung: Occludin

Hintergrund:

Synonyms: BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PTORCH1, Tight junction protein occludin

Gene Symbol: OCLN

Tissue Expression: Microvascular endothelial cells

Molekulargewicht: 60-82 kDa

Gen-ID: 100506658

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C