NEFH Antikörper (Phosphospecific) (CF®568)

Produktnummer ABIN7657903

Dieses Maus Monoklonal-Antikörper erkennt spezifisch NEFH in WB, IHC, FACS und IHC (fp). Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Huhn, Schwein, Rind (Kuh), Kaninchen, Meerschweinchen, Wüstenrennmaus und Cat.

Kurzübersicht für NEFH Antikörper (Phosphospecific) (CF®568) (ABIN7657903)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reaktivität: Human, Ratte, Maus, Huhn, Schwein, Rind (Kuh), Kaninchen, Meerschweinchen, Wüstenrennmaus, Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEFH Antikörper ist konjugiert mit CF®568

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: NE14

Produktdetails anti-NEFH Antikörper (CF®568)

Bindungsspezifität: Phosphospecific

Verwendungszweck: Neurofilament, phospho (NF-H) (Neuronal Marker)(NE14), CF568 conjugate

Produktmerkmale: This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). It reacts specifically with the phosphorylated KSP/KEP segment at the C-terminus of the heavy subunit (NF-H) of neurofilaments. After dephosphorylation of neurofilaments with alkaline phosphatase, this Ab no longer binds. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Immunogen: Crude neurofilament preparation from porcine spinal cord

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistochemistry (formalin-fixed): 0.25-0.5 μg/mL for 30 minutes at RT. Western blot: 1-2 μg/mL. Flow cytometry: 0.5-1 μg/million cells. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HEK293 cells, Brain, Neuroblastoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Andere Bezeichnung: Neurofilament H

Hintergrund:

Synonyms: NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200 kDa, Neurofilament Triplet H Protein, NF-H, NF200

Gene Symbol: NEFH

Tissue Expression: Neuroendocrine cells|Neurons

Molekulargewicht: 200 kDa

Gen-ID: 4744

UniProt: P12036