MUC5AC Antikörper (CF®740)

Produktnummer ABIN7657435

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MUC5AC in WB, IHC, FACS, ELISA und IHC (fp). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Affe, Cat, Kaninchen, Huhn, Schwein und Hedgehog.

Kurzübersicht für MUC5AC Antikörper (CF®740) (ABIN7657435)

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reaktivität: Human, Maus, Ratte, Affe, Cat, Kaninchen, Huhn, Schwein, Hedgehog

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC5AC Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: 45M1

Produktdetails anti-MUC5AC Antikörper (CF®740)

Verwendungszweck: Mucin 5AC (MUC5AC)(45M1), CF740 conjugate

Produktmerkmale: This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. The epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 0.5-1 μg/million cells in 0.1 mL. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology formalin-fixed 1-2 μg/mL. Does not react with cow, others not known. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Stomach

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu MUC5AC

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Andere Bezeichnung: Mucin 5AC

Hintergrund:

Synonyms: Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)

Gene Symbol: MUC5AC

Tissue Expression: Epithelial cells

Molekulargewicht: >1,000 kDa

Gen-ID: 4586

UniProt: P98088