MUC5AC Antikörper (CF®640R)

Produktnummer ABIN7657423

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MUC5AC in WB und RIA. Er zeigt eine Reaktivität gegenüber Human, Maus, Affe, Cat und Rind (Kuh).

Kurzübersicht für MUC5AC Antikörper (CF®640R) (ABIN7657423)

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reaktivität: Human, Maus, Affe, Cat, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MUC5AC Antikörper ist konjugiert mit CF®640R

Applikation: Western Blotting (WB), Radioimmunoassay (RIA)

Klon: 2-11M1

Produktdetails anti-MUC5AC Antikörper (CF®640R)

Verwendungszweck: MUC5AC (Mucin 5AC / Gastric Mucin)(2-11M1), CF640R conjugate

Produktmerkmale: This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). Its epitope is located in the N-terminal cysteine rich part of the peptide core of MUC5AC, which is heavily glycosylated. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. MAb 2-11M1 reacts with the protein backbone exclusively, it only reacts with fully deglycosylated MUC5AC. Therefore, the material under test should also be fully deglycosylated. This can be achieved with standard periodate oxidation method. The success of the deglycosylation can be checked with routine PAS (Periodic Acid Shiff) staining. After deglycosylation, the preparation should no longer be stainable with PAS reagent. Only then 2-11M1 will react should MUC5AC be present. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.

Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: ELISA: Use at 2-4 μg/mL for coating. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Western blot: 1-2 μg/mL. Flow cytometry: 0.5-1 μg/million cells. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: MCF-7 cells. Human colon or stomach (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu MUC5AC

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Andere Bezeichnung: MUC5AC

Hintergrund:

Synonyms: Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)

Gene Symbol: MUC5AC

Tissue Expression: Epithelial cells

Molekulargewicht: >1,000 kDa

Gen-ID: 4586

UniProt: P98088