TIMP2 Antikörper (AA 48-220)

Produktnummer ABIN7657007

Der Maus Monoklonal Anti-TIMP2-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, TIMP2 in Proben von Human zu detektieren.

Kurzübersicht für TIMP2 Antikörper (AA 48-220) (ABIN7657007)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TIMP2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: TIMP2-2044

Produktdetails anti-TIMP2 Antikörper

Bindungsspezifität: AA 48-220

Verwendungszweck: TIMP2 (Tissue Inhibitor of Metalloproteinase 2) (TIMP2/2044)

Produktmerkmale: This antibody recognizes a protein of 21 kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human TIMP2 protein fragment (aa 48-220)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. ELISA: 2-4 μg/m for coating order Ab without BSA. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HeLa cells. Normal Placenta. Breast, Colon, Endometrial, Prostate or Ovarian Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Andere Bezeichnung: TIMP2

Hintergrund:

Synonyms: Metalloproteinase inhibitor 2, TIMP metallopeptidase inhibitor 2, TIMP-2, Tissue Inhibitor of Metalloproteinase 2

Gene Symbol: TIMP2

Molekulargewicht: 21 kDa

Gen-ID: 7077

UniProt: P16035

Pathways: cAMP Metabolic Process