TIMP2 Antikörper (N-Term) (CF®740)

Produktnummer ABIN7656991

Dieses Maus Monoklonal-Antikörper erkennt spezifisch TIMP2 in IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Rind (Kuh), Zebrafisch (Danio rerio), Kaninchen und Meerschweinchen.

Kurzübersicht für TIMP2 Antikörper (N-Term) (CF®740) (ABIN7656991)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Zebrafisch (Danio rerio), Kaninchen, Meerschweinchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TIMP2 Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: 3A4

Produktdetails anti-TIMP2 Antikörper (CF®740)

Bindungsspezifität: AA 56-66, N-Term

Verwendungszweck: TIMP2 (Tissue Inhibitor of Metalloproteinase 2) (3A4), CF740 conjugate

Produktmerkmale: This antibody recognizes a protein of 21 kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin) 1-2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HeLa cells. Normal Placenta. Breast, Colon, Endometrial, Prostate or Ovarian Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Andere Bezeichnung: TIMP2

Hintergrund:

Synonyms: Metalloproteinase inhibitor 2, TIMP metallopeptidase inhibitor 2, TIMP-2, TIMP2, Tissue Inhibitor of Metalloproteinase 2

Gene Symbol: TIMP2

Molekulargewicht: 21 kDa

Gen-ID: 7077

UniProt: P16035

Pathways: cAMP Metabolic Process