MAML3 Antikörper (CF®740)

Produktnummer ABIN7656605

Der Maus Monoklonal Anti-MAML3-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, MAML3 in Proben von Human zu detektieren.

Kurzübersicht für MAML3 Antikörper (CF®740) (ABIN7656605)

Target: MAML3 (Mastermind-Like 3 (MAML3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAML3 Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: MAML3-1303

Produktdetails anti-MAML3 Antikörper (CF®740)

Verwendungszweck: MAML3 (Mastermind Like Transcriptional Coactivator 3) (MAML3/1303), CF740 conjugate

Produktmerkmale: MAML3 (mastermind-like protein 3) is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4.This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J, a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain of MAML3 is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, upregulation of MAML3 is thought to be involved in oncogenesis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length MAML3 protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Immunofluorescence 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: MCF-7 cells. Pancreas or Placenta. Cervix or Colon Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu MAML3

Target: MAML3 (Mastermind-Like 3 (MAML3))

Andere Bezeichnung: MAML3

Hintergrund:

Synonyms: CAGH3, ERDA3, GDN, MAML3, Mastermind-like protein 3, TNRC3

Gene Symbol: MAML3

Molekulargewicht: 150-170 kDa

Gen-ID: 55534