Maltose Binding Protein Antikörper (CF®594)

Produktnummer ABIN7656516

Dieses Anti-Maltose Binding Protein-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Maltose Binding Protein in WB. Geeignet für Diverse Spezies.

Kurzübersicht für Maltose Binding Protein Antikörper (CF®594) (ABIN7656516)

Target: Maltose Binding Protein (MBP)

Reaktivität: Diverse Spezies

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Maltose Binding Protein Antikörper ist konjugiert mit CF®594

Applikation: Western Blotting (WB)

Klon: R29-6

Produktdetails anti-Maltose Binding Protein Antikörper (CF®594)

Verwendungszweck: Maltose Binding Protein / MBP-probe(R29.6), CF594 conjugate

Produktmerkmale: Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage, such expression vectors frequently encode hybrid fusion proteins consisting in part of prokaryotic and in part, eukaryotic specified proteins. One such system utilizes maltose binding protein (MBP), the 370 amino acid product of the E. coli mal E gene. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be Purified in a one step procedure by affinity chromatography crosslinked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. Expression systems utilizing the MBP fusion tag include pCG-806fx and pMal vectors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: MOS maltose binding protein fusion protein.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL, Western blot 0.5-1.0 μg/mL, Optimal dilution for a specific application should be determined.

Kommentare:

Positive Control: Saos-2 cells. MBP fusion proteins.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Maltose Binding Protein

Target: Maltose Binding Protein (MBP)

Andere Bezeichnung: Maltose Binding Protein

Hintergrund:

Synonyms: ECK4026, JW3994, MalE, malJ, Maltodextrin binding protein, Maltose ABC transporter periplasmic protein, Maltose binding periplasmic protein, Periplasmic maltose binding protein

Gene Symbol: Not Known

Molekulargewicht: 40 kDa