Ku70/80 (Holo) Antikörper (CF®740)

Produktnummer ABIN7656310

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in IF und FACS. Er zeigt eine Reaktivität gegenüber Human und Non-Human Primate.

Kurzübersicht für Ku70/80 (Holo) Antikörper (CF®740) (ABIN7656310)

Target: Ku70/80

Reaktivität: Human, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: CF®740

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: KU729

Produktdetails

Bindungsspezifität: Holo

Verwendungszweck: Ku-Holo (p70,83)(KU729), CF740 conjugate

Produktmerkmale: Recognizes a dimer of two proteins of 70 kDa and ~80 kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Nuclear extract of human HL-60 cells

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunocytochemistry acetone-fixed cells 0.25-0.5 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Does not react with mouse, rat or chicken, others not known. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Human cancer K562, HL-60, HEp-2 or HeLa cells

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Antigendetails

Target: Ku70/80

Hintergrund:

Synonyms: Ku (p70): 70 kDa subunit of Ku antigen, ATP dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, Ku autoantigen, 70 kDa, Ku70, Kup70, Lupus Ku autoantigen protein p70, ML8, Thyroid autoantigen 70kD (Ku antigen), Thyroid-lupus autoantigen (TLAA), X-ray repair cross-complementing protein 6 (XRCC6) Ku (p80): 86 kDa subunit of Ku antigen, ATP dependent DNA helicase 2 subunit 2, ATP dependent DNA helicase II 86Kd subunit, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, KARP1, Ku autoantigen 80 kDa, Ku80, Ku86 autoantigen related protein 1, KUB2, Lupus Ku autoantigen protein p86, Nuclear factor IV (NFIV), Thyroid-lupus autoantigen (TLAA), X-ray repair cross-complementing protein 5 (XRCC5)

Gene Symbol: XRCC6

Molekulargewicht: 70 kDa & 80 kDa

Gen-ID: 2547