Keratin 7/17 Antikörper (CF®488A)

Produktnummer ABIN7656244

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Keratin 7/17 in IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human und Rind (Kuh).

Kurzübersicht für Keratin 7/17 Antikörper (CF®488A) (ABIN7656244)

Target: Keratin 7/17 (KRT7/17)

Reaktivität: Human, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Keratin 7/17 Antikörper ist konjugiert mit CF®488A

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: C-46

Produktdetails anti-Keratin 7/17 Antikörper (CF®488A)

Verwendungszweck: Cytokeratin 7/17(C-46), CF488A conjugate

Produktmerkmale: Recognizes intermediate filament proteins (IFP's) of 55 kDa and 46 kDa, which are identified as cytokeratin 7 and Cytokeratin 17 respectively. On formalin-fixed, paraffin embedded sections, C-46 reacts with keratin 7 only. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Cytokeratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Cytokeratin 17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. Cytokeratin 17 antibody marks basal cell differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Cytoskeletal preparation from HeLa cells (human cervical carcinoma cells)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: HeLa or ME-180 cells. Skin or hepatocellular carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Keratin 7/17

Target: Keratin 7/17 (KRT7/17)

Andere Bezeichnung: Keratin 7/17

Hintergrund:

Synonyms: CK-7; K2C7; Keratin 55K Type II Cytoskeletal; Keratin Simple Epithelial Type 1 K7; Keratin Type II Cytoskeletal 7; Krt2-7; KRT7; Sarcolectin; SCL; Type II Mesothelial Keratin K7; Type-II Keratin Kb7; CK17; K17; Keratin Type I Cytoskeletal 17; Keratin-17; KRT17; PCHC1

Gene Symbol: KRT7 & KRT17

Tissue Expression: Epithelial cells

Molekulargewicht: 55 kDa (CK7), 46 kDa (CK17)

Gen-ID: 3855, 3872

UniProt: P08729, Q04695