KRT17 Antikörper (CF®740)

Produktnummer ABIN7656020

Der Maus Monoklonal Anti-KRT17-Antikörper wurde für WB, IHC, IF, FACS und IHC (fp) validiert. Er ist geeignet, KRT17 in Proben von Human, Ratte, Rind (Kuh), Schwein und Ziege zu detektieren.

Kurzübersicht für KRT17 Antikörper (CF®740) (ABIN7656020)

Target: KRT17 (Keratin 17 (KRT17))

Reaktivität: Human, Ratte, Rind (Kuh), Schwein, Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KRT17 Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: E3

Produktdetails anti-KRT17 Antikörper (CF®740)

Verwendungszweck: Cytokeratin 17(E3), CF740 conjugate

Produktmerkmale: Cytokeratin 17 (CK17) is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glands such as mammary, sweat and salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. It may be considered asepithelial stem cellmarker because CK17 Ab marks basal cell differentiation. CK17 is expressed in SCLC much higher than in LADC. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-CK17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: The cytoskeletal fraction of rat colon epithelium

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology frozen & formalin-fixed 0.5-1 ug/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 ug/million cells/0.1 mL. Immunofluorescence 1-2 ug/mL. Western blotting 0.5-1 ug/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: T24 cells or Skin. Bladder or Cervix.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu KRT17

Target: KRT17 (Keratin 17 (KRT17))

Andere Bezeichnung: Keratin 17

Hintergrund:

Synonyms: K17, Keratin Type I Cytoskeletal 17, Keratin-17, KRT17, PCHC1

Gene Symbol: KRT17

Tissue Expression: Epithelial cells

Molekulargewicht: 46 kDa

Gen-ID: 3872

UniProt: Q04695