Rekombinanter KRT16 Antikörper (C-Term) (CF®740)

Produktnummer ABIN7655998

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch KRT16 in IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter KRT16 Antikörper (C-Term) (CF®740) (ABIN7655998)

Target: KRT16 (Keratin 16 (KRT16))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser KRT16 Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: KRT16-2043R

Produktdetails anti-KRT16 Antikörper (CF®740)

Bindungsspezifität: C-Term

Verwendungszweck: Cytokeratin 16 (KRT16) (Suprabasal Keratinocyte Marker) (KRT16/2043R), CF740 conjugate

Produktmerkmale: Cytokeratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I Cytokeratins (Cytokeratins 9 to 23) and one type II Cytokeratins (keratins 1 to 8). The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, to maintain the overall structural integrity of epithelial cells. Cytokeratins are also useful markers in identifying the origin of metastatic tumors. Cytokeratin 16 is expressed in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as luminal cells of mammary gland and sweat ducts. It is absent in non-invasive breast carcinomas and normal breast tissue.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment from the C-terminal of human Cytokeratin 16

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HeLa cells. Tonsil, Skin. Bladder, or Cervix.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu KRT16

Target: KRT16 (Keratin 16 (KRT16))

Andere Bezeichnung: Keratin 16

Hintergrund:

Synonyms: CK16, Cytokeratin-16, Focal non epidermolytic palmoplantar keratoderma (FNEPPK / NEPPK), K1CP, Keratin Type I Cytoskeletal 16, Keratin-16, KRT16A

Gene Symbol: KRT16

Tissue Expression: Epidermal cells

Molekulargewicht: 48 kDa

Gen-ID: 3868

UniProt: P08779