Rekombinanter Keratin 10 Antikörper (CF®647)

Produktnummer ABIN7655861

Der Maus Monoklonal Anti-Keratin 10-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, Keratin 10 in Proben von Human, Hund und Cat zu detektieren.

Kurzübersicht für Rekombinanter Keratin 10 Antikörper (CF®647) (ABIN7655861)

Target: Keratin 10 (KRT10)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Hund, Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Keratin 10 Antikörper ist konjugiert mit CF®647

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: RKRT10-1275

Produktdetails anti-Keratin 10 Antikörper (CF®647)

Verwendungszweck: Cytokeratin 10 (Suprabasal Epithelial Marker) (rKRT10/1275), CF647 conjugate

Produktmerkmale: This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-lenth human KRT10 protein.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: A431, HeLa, MCF7 cells. Esophagus or tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Keratin 10

Target: Keratin 10 (KRT10)

Andere Bezeichnung: Keratin 10

Hintergrund:

Synonyms: BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10

Gene Symbol: KRT10

Tissue Expression: Epidermal cells

Molekulargewicht: 56.5 kDa

Gen-ID: 3858

UniProt: P13645