Integrin beta 3 Antikörper (CF®740)

Produktnummer ABIN7655448

Dieses Anti-Integrin beta 3-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Integrin beta 3 in IHC und IHC (fp). Geeignet für Human.

Kurzübersicht für Integrin beta 3 Antikörper (CF®740) (ABIN7655448)

Target: Integrin beta 3 (ITGB3)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Integrin beta 3 Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: ITGB3-1713

Produktdetails anti-Integrin beta 3 Antikörper (CF®740)

Verwendungszweck: CD61 / Integrin beta-3 / Platelet Glycoprotein IIIa (Platelet Marker) (ITGB3/1713), CF740 conjugate

Produktmerkmale: This antibody reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). It associates with the alpha-V-chain (CD51) to form vitronectin receptor, or with the alpha-IIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61). The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human ITGB3 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow cytometry: 0.5-1 μg/million cells in 0.1 mL. Immunofluorescence: 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Immunohistology (frozen or formalin) 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: U937, KG1a, HEL cells. Spleen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Andere Bezeichnung: CD61

Hintergrund:

Synonyms: BDPLT2, GP3A, GPIIIa, GT, HPA 1, HPA 4, Integrin beta-3, ITGB3, NAIT, Platelet fibrinogen receptor beta subunit, Platelet glycoprotein IIIa, PTP

Gene Symbol: ITGB3

Tissue Expression: Platelets|Megakaryocytes

Molekulargewicht: 105 kDa & 90 kDa

Gen-ID: 3690

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex