Human Papilloma Virus 16 E2 (HPV-16 E2) Antikörper (Biotin)

Produktnummer ABIN7655171

Der Maus Monoklonal anti-Human Papilloma Virus 16 E2 Antikörper wird verwendet zum Nachweis von Human Papilloma Virus 16 E2 in Proben von Human Papillomavirus 16 (HPV-16). Er wurde validiert für WB und IF.

Kurzübersicht für Human Papilloma Virus 16 E2 (HPV-16 E2) Antikörper (Biotin) (ABIN7655171)

Target: Human Papilloma Virus 16 E2 (HPV-16 E2)

Reaktivität: Human Papillomavirus 16 (HPV-16)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: TVG 261

Produktdetails

Verwendungszweck: HPV16 E2 (Human Papilloma Virus 16)(TVG 261), Biotin conjugate

Produktmerkmale: The human papilloma virus (HPV) family of DNA tumor viruses includes HPV16, a strain that is responsible for the largest number of cases of cervical cancers linked to the family. HPV16E1 and HPV16E2 are proteins that are involved in the regulation of viral DNA replication and are important for infected cell homeostasis. HPV16E2 specifically regulates the expression of the E6 and E7 oncoproteins by binding to four sites within the viral long control region, possibly involving interactions with nuclear hormone receptors. Integration of the HPV genome into the host DNA usually disrupts the HPV16E2 gene open reading frames, resulting in an overexpression of E6 and E7 genes, an event that may lead to the malignant transformation of cervical cancer. HPV16E2 is also able to induce apoptotic cell death via two pathways: the first through the binding of p53 and the second through the binding of the viral genome. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Raised against Vaccinia-E2 followed by intravenous injection of the maltose binding protein MBP-E2

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.

Kommentare:

Positive Control: HPV-16 infected cells. Cervical tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Antigendetails

Target: Human Papilloma Virus 16 E2 (HPV-16 E2)

Andere Bezeichnung: HPV16 E2

Substanzklasse: Viral Protein

Hintergrund:

Synonyms: HPV16 regulatory protein E2, Human papilloma virus 16 E2, Human papilloma virus 16 regulatory protein E2, Human papillomavirus type 16 E2, Human papillomavirus type 16 regulatory protein E2, Regulatory protein E2

Gene Symbol: Not Applicable

Molekulargewicht: 43 kDa