FLI1 Antikörper (AA 107-261) (CF®740)

Produktnummer ABIN7654221

Dieses Anti-FLI1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von FLI1 in WB. Geeignet für Human.

Kurzübersicht für FLI1 Antikörper (AA 107-261) (CF®740) (ABIN7654221)

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FLI1 Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB)

Klon: FLI1-1312

Produktdetails anti-FLI1 Antikörper (CF®740)

Bindungsspezifität: AA 107-261

Verwendungszweck: FLI1 (Ewing s Sarcoma & Endothelial Marker) (FLI1/1312), CF740 conjugate

Produktmerkmale: This antibody recognizes a protein of 51 kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus.Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90 % of Ewing s Sarcoma (EWS)/Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11,22)(q24,q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi's Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (aa 107-261) of human FLI1 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: THP1 and Raji cells. Tonsil, Ewing s sarcoma (EWS) and Angiosarcoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu FLI1

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Andere Bezeichnung: FLI1

Hintergrund:

Synonyms: ERGB transcription factor, Ewing Sarcoma breakpoint region 2 (EWSR2), FLI1, FLI1 EWS fusion gene, Friend leukemia integration 1 (FLI1) transcription factor, Friend leukemia virus integration 1, Proto-oncogene Fli-1, SIC1, Transcription factor ERGB, Viral integration region FLI1

Gene Symbol: FLI1

Tissue Expression: Endothelial cells

Molekulargewicht: 51 kDa

Gen-ID: 2313

UniProt: Q01543